Electrophoresis Gel Stains
Dojindo Molecular Technologies Inc Nucleolus Bright Red (60 NMOL) allows for high localization in nucleolus, simply add the reagent to fixed cells to measure the senescence marker.
A Nucleolus is one of the structures forming a nucleus and becoming the starting point of the ribosomal biogenesis. There are many ribosomal RNA (rRNA) in the nucleolus and transcription of rRNA and processing are carried out. Whereas the morphological change of nucleolus is known as one of the indicators of cancer diagnosis, there have been some scientific reports describing the relation between nucleolus and DNA damage, autophagy, virus infection, and cellular senescence. Nucleolus Bright dyes are small molecules and they bind to RNA in the nucleolus to emit fluorescence. The nucleolus can be observed without any washing steps after staining with Nucleolus Bright dyes. We recommend to co-stain with DAPI in order to image the nucleolus clearly. Modern publications with a high number of citations summarize the various features of cellular senescence on the following page (https://www.dojindo.com/senescent-cell-detection-selection-guide/).
Biotang Inc PONCEAU S, 500 ML, ST-180, Ponceau S solution 0.1% (w/v) PONCEAU S in 5% acetic acid. Prepared in 18.2 megohms water and filtered through 1-micron filter. Shipped at room temperature. Store at room temperature.
PONCEAU S, 500 ML, ST-180, Ponceau S solution 0.1% (w/v) PONCEAU S in 5% acetic acid. Prepared in 18.2 megohms water and filtered through 1-micron filter. Shipped at room temperature. Store at room temperature.
Bioworld Rapid Coomassie Stain, 100 mL
Item# 21470008This stain is fast and sensitive in staining proteins in acrylimide gels. Its features include: a) completion within 30-60 minutes, b) unnecessary destaining, c) being highly sensitive, it detects as little as 30 ng of protein, d) yields sharper bands than those stained with conventional Coomassie Blue, and e) gels can be restained with Silver. Solution provided as 2x concentrate.
Sigma Aldrich Fine Chemicals Biosciences Thiazole Orange | 107091-89-4 | MFCD00192499 | 1g
Thiazole Orange | Mol Wt: 476.61 | 107091-89-4 | MFCD00192499 | 1g
Vector Laboratories Peanut Agglutinin (PNA) Rhodamine-labeled 5 mg
Peanut agglutinin (PNA) binds the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates and other soluble and membrane-associated glycoproteins and glycolipids. With exceptions, the PNA receptor sequence is normally sialylated, which prevents binding to its receptor oligosaccharide (see Jacalin). Sialic acid not bound to receptor sugars may also inhibit binding, and including calcium in diluents can enhance binding. This rhodamine-labeled PNA features a ratio of fluorophores to lectin protein that provides optimal staining (excitation 575 nm, emission 600 nm). It is supplied as a solution essentially free of unconjugated fluorophores.
Sigma Aldrich Fine Chemicals Biosciences Ethidium homodimer suitable for fluorescence, ~90% (HPCE) | 61926-22-5 | MFCD00210948 | 1MG
Ethidium homodimer suitable for fluorescence, ~90% (HPCE) | Purity: ~90% (HPCE) | Mol Wt: 856.75 | 61926-22-5 | MFCD00210948 | 1MG
Sigma Aldrich Fine Chemicals Biosciences Ethidium bromide ~95% (HPLC) | 1239-45-8 | MFCD00011724 | 25G
Ethidium bromide ~95% (HPLC) | Purity: ~95% (HPLC) | Mol Wt: 394.31 | 1239-45-8 | MFCD00011724 | 25G
Medchemexpress LLC Amaranth | 915-67-3 | 99.15% | 500 MG
Amaranth is an organic azo dye that inhibits cellular immune response. It can be used as a food additive.
- Inhibits cellular immune response
- Can be used as a food additive
Immunology Consultants anti-6xHIS Tag Antibody (Rabbit) - FITC Conjugated
This 0.1 mg FITC‑conjugated anti‑6xHIS antibody offers the same high‑quality fluorescent detection as the full‑size version in a smaller, economical format. Well suited for pilot experiments, optimization studies, or low‑volume imaging workflows, it provides bright FITC‑based visualization of HIS‑tagged proteins in microscopy and flow cytometry. Produced by Immunology Consultants, the antibody delivers strong epitope binding with minimal nonspecific signal. Its direct‑label design eliminates the need for secondary antibodies, enabling rapid, efficient detection in cell imaging and protein localization studies.
Thomas Scientific GelRed Nucleic Acid Gel Stain 10 000X in Water 0 1mL
GelRed Nucleic Acid Gel Stain 10 000X in Water 0 1mL